Journal: Burns & Trauma

Article Title: A multifunctional dihydromyricetin-loaded hydrogel for the sequential modulation of diabetic wound healing and glycemic control

doi: 10.1093/burnst/tkaf024

Figure Lengend Snippet: Immunomodulatory and anti-inflammatory properties of DPFI-n hydrogels. ( a ) Morphological changes in LPS-induced activated RAW 264.7 macrophages treated with DPFI-n hydrogel, demonstrating M1 to M2 polarization (scale bar: 50 μm). ( b ) Schematic representation of macrophages exhibiting different phenotypes. ( c and d ) Cell elongation and synaptic number changes of macrophages after different treatments. ( e and f ) Immunofluorescence imaging of iNOS-positive macrophages, with corresponding fluorescence intensity, highlighting reduced M1 markers following DPFI treatment (scale bar: 20 μm). ( g and h ) Immunofluorescence imaging of CD206-positive macrophages, along with corresponding fluorescence intensity, demonstrating increased M2 marker expression (scale bar: 20 μm). ( i – k ) PCR analysis of pro-inflammatory cytokine expression levels (IL-6, IL-1β, TNF-α) in macrophages after DPFI-n hydrogel treatment. ( l – n ) Expression levels of anti-inflammatory cytokines IL-4, IL-10, CD163 in macrophages after DPFI-n hydrogel treatment. * P <0.05, ** P <0.01, *** P <0.001, ns: no significance. Comparisons are made between the LPS group and other groups

Article Snippet: Co., Ltd Antibodies against iNOS and CD206 were obtained from Proteintech (USA).

Techniques: Immunofluorescence, Imaging, Fluorescence, Marker, Expressing